Remember, you're hoping the person who receives your letter has no memory of your closing at all. The last thing you want is a hiring manager going into an HR meeting with your cover letter in hand, asking the team if they want to meet with "Mr. Kindest Personal Regards."
Business writing varies from the conversational style often found in email messages to the more formal, legalistic style found in contracts. A style between these two extremes is appropriate for the majority of memos, emails, and letters. Writing that is too formal can alienate readers, and an attempt to be overly casual may come across as insincere or unprofessional. In business writing, as in all writing, you must know your audience.