Not only does this mean that you should focus on neutrality in your letter’s appearance (avoiding the use of colorful paper, brash logo designs, and artistic fonts), but it also means that you should employ a very low_key, unnuanced, and professional closing phrase. The best_case scenario is that the hiring manager, colleague, or connection won't even notice the closing.
In these days of email and instant messaging, letter writing is becoming a lost art, and especially the formal letter. I’m pretty sure that formal letter rules are still taught in schools, but very little of it sticks. If you’re an executive or other high_ranking person within an organization, you can probably rely on your secretary or assistant to handle the niceties of letter writing, but for everyone else, knowing how to write a strong letter ourselves is important.