Remember, you're hoping the person who receives your letter has no memory of your closing at all. The last thing you want is a hiring manager going into an HR meeting with your cover letter in hand, asking the team if they want to meet with "Mr. Kindest Personal Regards."
When you're writing a business letter or sending an email message, it's important to close your letter in a professional manner. The ideal ending for a business letter conveys your thanks and respect, without eccentricity or an overly familiar tone. Although it may seem old_fashioned, most business professionals expect written correspondence – whether via a letter or an email – to be written and formatted in a conservative manner.