If your intent is to persuade the recipient in some way, whether it's to invest money, give you a reference, hire you, partner with you, or fix an issue, create a compelling case for your cause. If, for example, you want the reader to sponsor a charity event, identify any overlap with their company’s philanthropic goals. Convince the reader that helping you would be mutually beneficial, and you will increase your chances of winning their support. Keep your closing paragraph to two sentences. Simply reiterate your reason for writing and thank the reader for considering your request.
If you’ve done that well, they’re going to be asking themselves “How can I make this happen?”, which you answer in the last section of your letter by proposing an action they can take. “Call me at 鴔) 555 to arrange an interview” or “Let’s meet next Thursday to discuss this proposal” or “Order your copy today”. Don’t leave the action for your recipient to figure out. I think a lot of time we lack the confidence to ask for the action we want, and we give ourselves an “out” by leaving it unsaid, hoping they’ll figure it out themselves.