When you know the recipient’s name, sign it “Sincerely yours” or just “Sincerely”, or in the US “Yours truly” is acceptable. Don’t try anything more cutesy or friendly unless a) you know the recipient quite well and are certain you can be informal without seeming disrespectful, or b) you have a “trademark” letter ending that’s part of your public persona (and the talent and popularity to pull it off). Signature and printed name: Signing your name is a minimal assurance that you stand behind what was written.
When writing an application letter, remember that you probably have competition. Your audience is a professional who screens and hires job applicants—someone who may look through dozens or even hundreds of other applications on the day she receives yours. The immediate objective of your application letter and accompanying resume is to attract this person’s attention. Your ultimate goal is to obtain an interview. As you write your application letter, be sure you complete three tasks: catch the reader’s attention favorably, convince the reader that you are a qualified candidate for the job, and request an interview.